Production of Transgenic Rats via Lentiviral Transduction and Xenogeneic Transplantation of Spermatogonial Stem Cells1
نویسندگان
چکیده
منابع مشابه
Production of transgenic rats via lentiviral transduction and xenogeneic transplantation of spermatogonial stem cells.
Spermatogonial stem cells (SSCs) continue to proliferate in the testis to support spermatogenesis throughout life, which makes them ideal targets for germline modification. Although recent success in the production of transgenic and knockout animals using SSCs has opened up new experimental possibilities, several problems, including the low efficiency of germ cell transplantation and poor ferti...
متن کاملProduction of transgenic rats by lentiviral transduction of male germ-line stem cells.
Primary cultures of rat spermatogenic cells that did not bind to collagen matrices were able to colonize and form mature spermatozoa when transferred to testes of recipient males. Up to 73% of the progeny from matings with recipient males were derived from the transferred spermatogenic cells. Subsequently, two populations of germ cells were obtained by selection on laminin matrices. Both popula...
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nitric oxide (no) is a small molecule synthesized by most of mammalian cells with diverse biological activities including vasodilatation, host defense and wound healing. impaired wound healing is a common occurrence among diabetics and patients receiving glucocorticoid therapy. on the other hand the application of laser in biomedical area have been increased. thus, the current studies were desi...
15 صفحه اولSpermatogonial stem cell isolation, storage, and transplantation.
The past 30 years have been marked by unparalleled accomplishments in the medical treatment of malignancy. Prior to advances in chemotherapeutic and radiation treatment, many oncologic conditions had dismal survival rates. Today, medical interventions have success rates that approach complete remission for many malignancies. An inadvertent complication of these therapies, however, has been the ...
متن کاملLentiviral vector-mediated transduction of adult neural stem/progenitor cells isolated from the temporal tissues of epileptic patients
Objective(s): Neural stem/progenitor cells (NS/PCs) hold a great potential for delivery of therapeutic agents into the injured regions of the brain. Efficient gene delivery using NS/PCs may correct a genetic defect, produce therapeutic proteins or neurotransmitters, and modulate enzyme activation. Here, we investigated the efficiency of a recombinant lentivirus vector ...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 2008
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod.108.071159